Table 1

The oligonucleotide sequence of primers used for detecting expression of genes of interest, in the RT-PCR assay.

Oligonucleotide

Sequence

Amplified length

Annealing temperature


PGM1–1718

5'-TCCGACTGAGCGGCACTGGGAGTGC-3'

386bp

63°C

PGM1–2080

5'-TCCGACTGAGCGGCACTGGGAGTGC-3'

hTR-F

5'-CGCCGTGCTTTTGCT CC-3'

316bp

63°C

hTR-R

5'-ACTCGCTCCGTTCCTCTTCC-3'

hTERT–13187

5'-CGG AAG AGT GTC TGG AGC AA-3'

145bp

67°C

hTERT–15411

'5'-TCC AGA CTC CGC TTC ATC C-3'


Hosseini-Asl et al. International Seminars in Surgical Oncology 2006 3:20   doi:10.1186/1477-7800-3-20

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