Table 2 |
|||||
|
Primer sequences for exons 2, 3, & 13 |
|||||
|
BRCA1 |
EXON |
PRIMER Sequences(5'-to-3') |
Product size |
Tm(°C) |
|
|
|
|||||
|
EXON2 |
F |
GGTTGTGATTAGTTCTTTGG |
458 |
51.3 |
|
|
R |
GTGTTGAAAAGGAGAGGAGT |
53.4 |
|||
|
EXON3 |
R |
GAATGAAATGGAGTTGGATT |
381 |
55.81 |
|
|
F |
AGGATCGTATTCTCTGCTGT |
53.98 |
|||
|
EXON13 |
R |
AGAACCAAGGCTCCATAAT |
476 |
54 |
|
|
F |
ATTGCATGAATGTGGTTAGA |
53.76 |
|||
|
|
|||||
|
Malik et al. International Seminars in Surgical Oncology 2008 5:21 doi:10.1186/1477-7800-5-21 |
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